Journal: Cell reports
Article Title: BCL2 drives castration resistance in castration-sensitive prostate cancer by orchestrating reciprocal crosstalk between oncogenic pathways
doi: 10.1016/j.celrep.2025.115779
Figure Lengend Snippet: (A) Western blots (WB) of indicated proteins in LNCaP cells treated with increasing concentration R1881 under ADT (CSS) for 72 h. (B) Schematic representation of the experimental design for AR regulation of BCL2 expression. (C) WB of LNCaP cells either treated with 1 nM R1881 alone or in combination with increasing concentration (0.03–10 μM) of ENZA normalized to DMSO for 48 h. (D) WB of LAPC4 cells either treated with 1 nM R1881 alone or in combination with indicated concentration of ENZA normalized to DMSO for 48 h. (E) WB of LNCaP cells either treated with 1 nM R1881 alone or in combination with indicated concentration of apalutamide or darolutamide normalized to DMSO for 48 h. (F) WB of LNCaP cells either transfected with control siRNA or AR siRNA for 72 h. Post-transfected cells are cultures either in complete medium or under ADT (CSS) alone for 2 days or CSS supplemented with 1 nM R1881 for (first day in CSS, second day in CSS + R1881). (G) qPCR analysis of 2-month-old normal mouse prostate and castrated mouse prostate (15 days post castration) for bcl2 and bclxl mRNA expression. Error bar, SD. (H) qPCR analysis of indicated genes in the LNCaP cells transiently transfected (overexpression [OE]) for 72 h with full-length AR or AR-V7 or GFP (control). Post-transfected cells were cultured in complete medium. Error bar, SD. (I) WB of LNCaP cells treated with indicated concentration of PU-h71 normalized to DMSO for 48 h. (J) WB of LNCaP-Abl cells treated with 1 nM R1881 alone or in combination with 0.10 μM ENZA normalized to DMSO for 48 h. (K) Schematic model of BCL2 expression regulation in CSPC vs. CRPC. WBs and qPCRs are representatives of n = 3 technical replicates.
Article Snippet: Human AR V7: pEGFP-C1-AR , Addgene , Gift from Michael Mancini & Marco Marcelli (Addgene plasmid # 86856 http://n2t.net/addgene:86856 ; RRID:Addgene_86856).
Techniques: Western Blot, Concentration Assay, Expressing, Transfection, Control, Over Expression, Cell Culture
Addgene inc is a verified supplier 
![(A) Western blots (WB) of indicated proteins in LNCaP cells treated with increasing concentration R1881 under ADT (CSS) for 72 h. (B) Schematic representation of the experimental design for AR regulation of BCL2 expression. (C) WB of LNCaP cells either treated with 1 nM R1881 alone or in combination with increasing concentration (0.03–10 μM) of ENZA normalized to DMSO for 48 h. (D) WB of LAPC4 cells either treated with 1 nM R1881 alone or in combination with indicated concentration of ENZA normalized to DMSO for 48 h. (E) WB of LNCaP cells either treated with 1 nM R1881 alone or in combination with indicated concentration of apalutamide or darolutamide normalized to DMSO for 48 h. (F) WB of LNCaP cells either transfected with control siRNA or AR siRNA for 72 h. Post-transfected cells are cultures either in complete medium or under ADT (CSS) alone for 2 days or CSS supplemented with 1 nM R1881 for (first day in CSS, second day in CSS + R1881). (G) qPCR analysis of 2-month-old normal mouse prostate and castrated mouse prostate (15 days post castration) for bcl2 and bclxl mRNA expression. Error bar, SD. (H) qPCR analysis of indicated genes in the LNCaP cells transiently transfected (overexpression [OE]) for 72 h with full-length AR or <t>AR-V7</t> or GFP (control). Post-transfected cells were cultured in complete medium. Error bar, SD. (I) WB of LNCaP cells treated with indicated concentration of PU-h71 normalized to DMSO for 48 h. (J) WB of LNCaP-Abl cells treated with 1 nM R1881 alone or in combination with 0.10 μM ENZA normalized to DMSO for 48 h. (K) Schematic model of BCL2 expression regulation in CSPC vs. CRPC. WBs and qPCRs are representatives of n = 3 technical replicates.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6453/pmc12316453/pmc12316453__nihms-2092657-f0003.jpg)